Note that you can also browse all contigs with SNPs and blast the EST contig database.

A tool to download individual assembled EST contigs is also available.

1. Please Choose: Help

• Enter a Pfam Accession # (e.g.: PF00515)
• Enter a Motif Name or Protein Description Keyword (e.g.: TPR_1)
• Browse / Search directory to find a family of interest.

Pfam accession   OR  Protein Family Name   OR StellaBase ID #   

2. Please Choose an e-value threshold: (Note: This e-value threshold will limit the number of EST contigs which match a specific PFAM motif) Help

1e-100 1e-90 1e-80 1e-70 1e-60 1e-50 1e-40 1e-30 1e-20 1e-10 1e-9 1e-8 1e-7 1e-6 1e-5 1e-4 1e-3 1e-2 1e-1 1 10

3. Minimum Redundancy = the number of polymorphic bases at a base position. For example, assume that there are 10 distinct ESTs for GeneX. Assume that either a C, T, or G may be found at position n of GeneX. If C is found at position x in 8 ESTs, and T and G are each found at position x in 1 EST then the Miminum Redunancy is 3.

7 6 5 4 3 2 1

4. Select only those cDNAs which have at least this number of SNPs which match my redundancy threshold.

50 30 20 15 10 9 8 7 6 5 4 3 2 1

5. Submit Your Query:

Please Note: This search may take several seconds or minutes, particularly if you search using a PFAM Accession #.